eclipse e800 epifluorescence microscope Search Results


eclipse e800 epifluorescence microscope  (Nikon)
99
Nikon eclipse e800 epifluorescence microscope
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Eclipse E800 Epifluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e800 epifluorescence microscope 188  (Nikon)
99
Nikon e800 epifluorescence microscope 188
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
E800 Epifluorescence Microscope 188, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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c1 confocal microscopy attachment  (Nikon)
99
Nikon c1 confocal microscopy attachment
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
C1 Confocal Microscopy Attachment, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transmitted epifluorescence light microscope  (Nikon)
96
Nikon transmitted epifluorescence light microscope
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Transmitted Epifluorescence Light Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+e800+epifluorescence+microscope/Fluorescence+Filter+Cubes/pm31301204-80-5-8
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nikon e800 upright microscope  (Nikon)
93
Nikon nikon e800 upright microscope
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Nikon E800 Upright Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nikon e800 epifluorescence microscope  (Image Systems Inc)
90
Image Systems Inc nikon e800 epifluorescence microscope
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Nikon E800 Epifluorescence Microscope, supplied by Image Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+e800+epifluorescence+microscope/nikon+eclipse+e800/10__1074_slash_jbc__m408273200-83-8-11
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metamorph software  (MetaMorph Inc)
90
MetaMorph Inc metamorph software
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Metamorph Software, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+e800+epifluorescence+microscope/metamorph+software/10__1128_slash_jb__186__14__4492___4501__2004-112-14-9
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eclipse e800 microscope  (Nikon)
95
Nikon eclipse e800 microscope
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Eclipse E800 Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eclipse+e800+epifluorescence+microscope/Maximum+Magnification+Module/pmc03641229-398-18-17
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nikon eclipse e800 microscope  (Bergman Labora AB)
86
Bergman Labora AB nikon eclipse e800 microscope
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Nikon Eclipse E800 Microscope, supplied by Bergman Labora AB, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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orcaflash 4.0 camera  (Hamamatsu)
90
Hamamatsu orcaflash 4.0 camera
Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the <t>epifluorescence</t> microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.
Orcaflash 4.0 Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the epifluorescence microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.

Journal: Journal of the American Society of Nephrology

Article Title: Calcitonin Has a Vasopressin-like Effect on Aquaporin-2 Trafficking and Urinary Concentration

doi: 10.1681/asn.2009121267

Figure Lengend Snippet: Figure 5. Immunogold electron microscopy shows apical plasma membrane insertion of AQP2 induced by CT in vivo. AQP2 was localized by pre-embedding labeling of thick, nonperme- abilized vibratome sections kidney cortical tissue with an antibody against an external epitope of AQP2. Only plasma membrane AQP2 is de- tected using this procedure. CT treatment (B) showed a significant amount of AQP2 plasma membrane associated with the apical membrane and microvilli, whereas AQP2 in the apical plasma membrane was much less abundant in cortical kidney sections of the untreated rats (A). These results support the epifluorescence microscopy data shown in Figures 6 and 9. The number of gold particles labeling AQP2 is expressed per micrometer of apical membrane length (C). Images of two to three tubules from each tissue were analyzed with ImageJ software (National Institutes of Health). The density of AQP2 at the apical plasma membrane (open bar) was compared with the density of AQP2 in the apical membranes of untreated rats (solid bar) (means SEM; n 3; *P 0.05). The position of the cell junction between a principal cell (B) and an AQP2-negative in- tercalated cell (A) is indicated with an arrow in each figure. The bar indicates 0.5 m.

Article Snippet: Mounted slides were examined using a Nikon Eclipse E800 epifluorescence microscope equipped with a 40 1.0NA Plan Apo objective, and the images were captured digitally using a Hamamatsu Orca CCD camera and IPLab Spectrum software (Scanalytic, Vienna, VA).

Techniques: Electron Microscopy, Clinical Proteomics, Membrane, In Vivo, Labeling, Epifluorescence Microscopy, Software